Calsequestrin

Calsequestrin

Description: Calsequestrin is the major calcium storage protein of the sarcoplasmic reticulum. Intraluminar Ca2+ binds to calsequestrin during diastole to prevent Ca2+ precipitation and to lower its free ionic concentration to facilitate efficient storage. During systole, Calsequestrin coordinately releases ~4050 Ca2+ ions per molecule for each contraction-relaxation cycle by an uncertain mechanism. Calsequestrin has been shown to be a major importance in the regulation of cardiac excitation-contraction coupling.

Source: Dog Heart.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-HCL and 1mM EGTA.

Solubility: It is recommended to reconstitute the lyophilized Calsequestrin in sterile 18M?-cm H2O not less than 100 ?g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Calsequestrin although stable at room temperature for 3 weeks, should be stored desiccated below -18�C. Upon reconstitution Calsequestrin should be stored at 4�C between 2-7 days and for future use below -18�C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 90% as determined by: SDS-PAGE

Applications: Immunoblots. Absorption. Immunohistochemistry

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.