Cell Division Cycle 42 Human (Recombinant)

Synonyms

Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42, G25K, CDC42Hs.

CDC42 (Cell division cycle 42 isoform 1) is a small GTPase of the Rho-subfamily that regulates signaling pathways which control various cellular functions including cell morphology, migration, endocytosis and cell cycle progression. CDC42 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In the active state, CDC42 binds to a variety of effector proteins to regulate cellular responses. CDC42 is involved in epithelial cell polarization processes. CDC42 causes the formation of thin, actin-rich surface projections called filopodia. Also, CDC42 regulates actin polymerization through its direct binding to N-WASP (Neural Wiskott-Aldrich syndrome protein), which subsequently activates Arp2/3 complex. Loss of CDC42 function raises endocytotic uptake of apical proteins, as well as apical polarity factors such as Crumbs. The product of oncogene Dbl was described to specifically catalyze the dissociation of GDP from CDC42 protein.

Description

CDC42 Human Recombinant fused with a 15 amino acid T7 tag at N-terminus produced in E. coli is a single, non-glycosylated, polypeptide chain containing 203 amino acids (1-188 a.a.) and having a molecular mass of 22.4kDa. The CDC42 is purified by proprietary chromatographic techniques.

Source

Escherichia coli

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The CDC42 solution contains 20mM Tris-HCl buffer (pH8.0), 1mM DTT, 10% glycerol and 2mM EDTA.

Stability

CDC42 although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Amino acid sequence

MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC.

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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CDC25A Human, CDC26 Human, CDC34 Human, CDC37 Human, CDC37 Human, His