Cell Division Cycle Associated 8 Human (Recombinant)

Synonyms

Cell division cycle associated 8, BOR, hDasraB, FLJ12042, MESRGP, Pluripotent embryonic stem cell-related gene 3 protein, PESCRG3, BOREALIN.

CDCA8 is a component of the chromosomal passenger complex (CPC). The CPC complex has crucial functions at the centromere in ensuring accurate chromosome placement and isolation and is obligatory for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 protein is needed to direct the CPC to centromeric DNA. Removal of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach.

Description

CDCA8 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E. coli is a single, non-glycosylated, polypeptide chain containing 283 amino acids (19-280a.a.) and having a molecular mass of 31.6kDa. The CDCA8 is purified by proprietary chromatographic techniques.

Source

Escherichia coli

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The CDCA8 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 50% glycerol, 1mM DTT, 1mM EDTA and 0.2M NaCl.

Stability

CDCA8 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAA TADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRL EVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSN RLAQICSSIR THK

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.