C-JUN (Recombinant)

Recombinant Human C-JUN

Source: E.Coli

Background: C-JUN is a gene which, in combination with c-Fos, forms the AP-learly response transcription factor. C-JUN is activated by the JNK pathway. C-JUN is the putative transforming gene of avian sarcoma virus 17. C-JUN is a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA swquences to regulate gene expression. The C-JUN gene is intronless and is mapped to lp32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Description: Recombinant Human C-JUN amino acids 1-81 produced in E.coli is a non-glycosylated, polypeptide chain having a molecular mass of 52 KD. Recombinant C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant Human C-JUN is supplied as lyophilized powder containing no additives.

Soubility: It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-JUN is recommened in 40mM Tris, pH7.5, to a concentration of 0.2-1.0 mg/ml.

Biological Activity: Recombinant human c-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application. Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Storage: Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 95% (SDS-PAGE).

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.