Coagulation Factor-VIII Human (Recombinant)

Synonyms

Coagulation factor VIII, Procoagulant component, Antihemophilic factor, AHF, F8, F8C, F8B, HEMA, FVIII, DXS1253E, F8 protein.

Coagulation factor VIII participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Description

Antihemophilic Facor Human Recombinant produced in CHO is a glycosylated polypeptide chain having 2322 amino acids. The Factor-VIII is purified by proprietary chromatographic techniques.

Source

CHO cells (Chinese Hamster Ovarian Cells).

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Reconstitution of 250IU vial in 5ml distilled water will contain 8mg Tween-80, 112mM NaCl, 40mg Mannitol, 10mg Trehalose, 1ng VWF and 4.2mM CaCl2.

Solubility

It is recommended to reconstitute the lyophilized Factor-VIII in 1ml sterile 18MΩ-cm H2O, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Factor-VIII although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution Factor-VIII should be stored at 2-8°C between 2-7 days and for future use below -20°C. Please prevent freeze-thaw cycles.

Biological Activity

The specific activity was found to be 7,058 IU/mg.

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

F7 Human, F8 Human, F9 Antibody, F9 Human