Complement C1q Tumor Necrosis Factor-Related Protein 8 Human (Recombinant)

Synonyms

CTRP8, UNQ5829, C1q/TNF-related protein 8, Complement C1q tumor necrosis factor-related protein 8.

Description

C1QTNF8 Protein is a 28.4 kDa protein containing 255 aa fused to a 10 aa N-Terminal His-tag.

Source

E. coli

Formulation

C1QTNF8 Human was filtered (0.4µm) and lyophilized from 0.5mg/ml supplied in 0.03M Acetate buffer, pH 4.

Solubility

Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10?g/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Stability

Store lyophilized C1QTNF8 uman at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted C1QTNF8 an be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino Acid Sequence

MKHHHHHHAS WPGLPRRPCV HCCRPAWPPG PYARVSDRDL WRGDLWRGLP RVRPTIDIEI LKGEKGEAGV RGRAGRSGKE GPPGARGLQG RRGQKGQVGP PGAACRRAYA AFSVGRRAYA AFSVGRREGL HSSDHFQAVP FDTELVNLDG AFDLAAGRFL CTVPGVYFLS LNVHTWNYKE TYLHIMLNRR PAAVLYAQPS ERSVMQAQSL MLLLAAGDAV WVRMFQRDRD NAIYGEHGDL YITFSGHLVK PAAEL

Quality Control Test

BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.

Applications

Western blotting

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.