Cystatin 9 Human (Recombinant)

Synonyms

CLM, cystatin-9 (testatin), Cystatin-like molecule.

CST9 belongs to the CRES (cystatin-related epididymal spermatogenic) subfamily. The CRES (cystatin-related epididymal spermatogenic) protein outlines a new subgroup in the family 2 cystatins of the cystatin superfamily. A few members are active cysteine protease inhibitors, however others have lost or possibly never developed this inhibitory activity. CST9 takes part in hematopoietic differentiation or inflammation and is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas. CST9 is upregulated by LPS in several cancer cell lines, such as promyelocytic leukemia (HL-60) and myelomonocytic leukemia.

Description

CST9 Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (29-159) and having a molecular mass of 17.2 kDa. CST9 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

E. coli

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The CST9 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MWCSEEEMGG NNKIVQDPMF LATVEFALNT FNVQSKEEHA YRLLRVLSSW REDSMDRKWR GKMVFSMNLQ LRQTVCRKFE DDIDNCPFQE SLELNNVRQG ISFPQVHSCG CCMGCGVGTG AADKAIPRDK GK

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.