Cytomegalo Virus Pp150(UL32), (Recombinant)

Recombinant CytomegaloVirus phosphoprotein 150 (pp150)

Source: E.Coli

Background: CMV belongs to the Betaherpesvirinae subfamily of Herpesviridae which includes herpes simplex virus types 1 and 2, varicella-zoster virus, and Epstein-Barrvirus. The herpes viruses share a characteristic ability to remain latent over long periods. CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane. CMV has the largest genome of the herpes viruses, ranging from 230-240 kilobase pairs. Human CMV is composed of unique and inverted repeats that include the existence of 4 genome isomeers caused by inversion of L-S genome components (class E). Replication may be divided into immediate early , delayed early, and late gene expression based on time of synthesis after infection. The DNA is replicated by rolling circles. In vitro, CMV replicates in human fibroblasts.

Description: The E.Coli derived recombinant protein contains the CMV Pp150 (UL32) immunodominant regions, 1011-1048 amino acids.

Purification method: Purified by proprietary chromatographic technique.

Purity: Recombinant CMV-pp150 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation: 1mg/ml in 25 mM Tris-Hcl pH 8.0, 1 mM EDTA and 50% glycerol.

Storage: Recombinant CMV-pp150 is shipped at ambient temperature. Upon arrival, Store at -20°C.

Stability: Recombinant CMV-pp150 is stable 5 years frozen, One month in solution at room temperature.

Specificity: Immunoreactive with sera of CMV-infected individuals

Application: Recombinant CMV-pp150 Antigen may be used in ELISA and Western blots, excellent for detection of CMV with minimal specificity problems.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.