Der P1 Protein (Recombinant)

Synonyms: Peptidase 1, Major mite fecal allergen Der p 1, Allergen Der p I, Der p 1, DERP1, Der-P1

DERP1 is a thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues. DERP1 is a C1 peptidase family member. DERP1 has extensive endopeptidase specificity. DERP1 is N-glycosylated. N-glycanase treatment does not completely remove carbohydrates, suggesting that the protein contains additional glycosylation sites. DERP1 causes an allergic reaction in humans. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis. DERP1 binds to IgE in 80% of patients with house dust allergy.

Description: The E. coli derived recombinant protein contains the Dermatophagoides pteronyssinus Der P1 full length protein (a.a. 1-321) and fused to a 6 His Tag at C-terminus, having a total Mw of 34.5kDa, pI 5.6.

Method: Purified by proprietary chromatographic technique.

Formulation: (1mg/ml) 60mM NaCl, 50mM Tris-HCl pH 8.0 and 1.2M Urea.

Storage: Protein is shipped at ambient temperature. Upon arrival, Store at -20°C .

Stability: Five years frozen. One month in solution at room temperature.

Applications: Use as an antigen in ELISA and Western Blots.

Usage: Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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