Disulfide Oxidoreductase (Recombinant)

Disulfide oxidoreductase (DsbA)

Source: E.Coli

Background: DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli. dsbA is purified by proprietary chromatographic techniques.

Description: DsbA produced in E.Coli is a periplasmic protein isolated from E. coli having a molecular mass of 23,149 Dalton dsbA is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: dsbA was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride..

Solubility: It is recommended to reconstitute the lyophilized Human dsbA in sterile 18MΩ-cm H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized dsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution dsbA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) SDS-PAGE

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ly-Lys-Ala-Trp.

Applications: 1.Western blot (we recommend that the material be diluted in 1X SDS-PAGE sample buffer (1). On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a colorimetric Western Blot with D9625 at a dilution of 1: 10,000 as the primary antibody and an appropriate alkaline phosphatase conjugated secondary antibody for detection.)

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.