DNA Damage Inducible Transcript 4 (Recombinant) Human

Synonyms

DNA-damage-inducible transcript 4, RTP801, REDD-1, Dig2, FLJ20500, Protein regulated in development and DNA damage response 1, HIF-1 responsive protein RTP801, RP11-442H21.1.

DDIT4 has a role in the regulation of reactive oxygen species. DDIT4 is upregulated at the transcriptional level as a reaction to stress due to DNA damage and glucocorticoid treatment. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase frequently referred to as mTOR. DDIT4 is vital in the coupling of extra- and intracellular cues to mTOR regulation.

Description

DDIT4 produced in E. coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-232.a.a) and having a molecular mass of 27.5kDa. DDIT4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia coli

Physical Appearance

Sterile Filtered clear solution.

Formulation

The DDIT4 protein solution (0.25mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and 30% glycerol.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.