DnaJ (Hsp40) Homolog, Subfamily B, Member 11 Human (Recombinant)

Synonyms

DnaJ homolog subfamily B member 11, APOBEC1-binding protein 2, ABBP-2, DnaJ protein homolog 9, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, ER-associated dnaJ protein 3, ERdj3, ERj3p, HEDJ, Human DnaJ protein 9, hDj-9, PWP1-interacting protein 4, DNAJB11, EDJ, ERJ3, HDJ9, DJ9, Dj-9, ABBP2, UNQ537, PRO1080.

DNAJB11 is a member of the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJB11 serves as a co-chaperone for HSPA5 and binds directly to both unfolded proteins which are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed.

Description

DNAJB11 produced in E. coli is a single, non-glycosylated polypeptide chain containing 357 amino acids (23-358 a.a.) and having a molecular mass of 40.5kDa. DNAJB11 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia coli

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The DNAJB11 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGRDFYKILG VPRSASIKDI KKAYRKLALQ LHPDRNPDDP QAQEKFQDLG AAYEVLSDSE KRKQYDTYGE EGLKDGHQSS HGDIFSHFFG DFGFMFGGTP RQQDRNIPRG SDIIVDLEVT LEEVYAGNFV EVVRNKPVAR QAPGKRKCNC RQEMRTTQLG PGRFQMTQEV VCDECPNVKL VNEERTLEVE IEPGVRDGME YPFIGEGEPH VDGEPGDLRF RIKVVKHPIF ERRGDDLYTN VTISLVESLV GFEMDITHLD GHKVHISRDK ITRPGAKLWK KGEGLPNFDN NNIKGSLIIT FDVDFPKEQL TEEAREGIKQ LLKQGSVQKV YNGLQGY

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

DNAJB2 Human, DNAJB6 Human, DNAJB8 Human