E.Coli GroES (HSP10), (Recombinant)

Synonyms

CPN10, GROES, HSP10, HSPE1, 10 kDa chaperonin, Protein Cpn10, groES protein, 11.2 kDa stress response protein, Heat shock protein 10.

GroES protein is the co-chaperonin of GroEL in E. coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques.

Description

Recombinant GroES produced in E. coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 10.4 kDa.

Source

Escherichia coli

Physical Appearance

Sterile filtered colorless solution.

Formulation

The groES protein contains 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 1mM DTT and 10% Glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Amino Acid sequence

MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE GEVKPLDVKVGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

GroES Human, GroES Human, His