Heat Shock 70kDa protein 1B Human (Recombinant)

Synonyms

Heat shock 70kDa protein 1B, HSP70-2, HSP70-1/HSP70-2, FLJ54328, HSPA1A, heat shock 70kDa protein 1A/1B, HSPA1.

The HSP70 family is found in many intracellular compartments such as chloroplasts, endoplasmic reticulum, mitochondria, and cytosol. These proteins are induced by a variation of biological stresses, including heat stress, in every organism. HSP70 has a range of functions. For example: HSP70 acts as molecular chaperones facilitating the assembly of multi-protein complexes, HSP70 take part in the translocation of polypeptides across cell membranes and to the nucleus, and HSP70 assists in the proper folding of nascent polypeptide chains.
HSP70 is a mitochondrial import equipment and has a crucial part in the cytosolic endoplasmic reticulum. Lately, extracellular localized HSP was discovered to take an important part in the induction of a cellular immune response.

Description

The HSPA1B Protein Human produced in E. coli is a single, non-glycosylated polypeptide chain containing 650 amino acids having a molecular mass of 71.16kDa. HSPA1B is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Escherichia coli

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was filtered (0,4µm) and lyophilized from a 0.5mg/ml solution containing 50mM phosphate buffer, 75mM NaCl, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized HSPA1B in sterile H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized HSPA1B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSPA1B should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Amino Acid Sequence

MKHHHHHHAS AKAAAIGIDL GTTYSCVGVF QHGKVEIIAN DQGNRTTPSY VAFTDTERLI GDAAKNQVAL NPQNTVFDAK RLIGRKFGDP VVQSDMKHWP FQVINDGDKP KVQVSYKGET KAFYPEEISS MVLTKMKEIA EAYLGYPVTN AVITVPAYFN DSQRQATKDA GVIAGLNVLR IINEPTAAAI AYGLDRTGKG ERNVLIFDLG GGTFDVSILT IDDGIFEVKA TAGDTHLGGEDFDNRLVNHF VEEFKRKHKK DISQNKRAVR RLRTACERAK RTLSSSTQAS LEIDSLFEGI DFYTSITRAR FEELCSDLFR STLEPVEKAL RDAKLDKAQI HDLVLVGGST RIPKVQKLLQ DFFNGRDLNK SINPDEAVAY GAAVQAAILM GDKSENVQDL LLLDVAPLSL GLETAGGVMT ALIKRNSTIP TKQTQIFTTY SDNQPGVLIQ VYEGERAMTK DNNLLGRFEL SGIPPAPRGVPQIEVTFDID ANGILNVTAT DKSTGKANKI TITNDKGRLS KEEIERMVQE AEKYKAEDEV QRERVSAKNA LESYAFNMKS AVEDEGLKGK ISEADKKKVL DKCQEVISWL DANTLAEKDE FEHKRKELEQ VCNPIISGLY QGAGGPGPGG FGAQGPKGGS GSGPTIEEVD

Applications

Western blotting, ELISA.

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.