Heat Shock Protein 22 kDa Protein-8 Human (Recombinant)

Synonyms

HSPB8, H11, HMN2, CMT2L, DHMN2, E2IG1, HMN2A, HSP22, Heat shock protein beta-8, Alpha-crystallin C chain, Small stress protein-like protein HSP22, E2-induced gene 1 protein, Protein kinase H11, CRYAC.

HSPB8 displays temperature-dependent chaperone activity. HSPB8 acts as a Mn(2+)-dependent serine-threonine- specific protein kinase. We are not convinced that this is its true role. Defects in HSPB8 are a cause of distal hereditary motor neuropathy type ii (DHMN2) also known as distal spinal muscular atrophy (DSMA) and spinal muscular atrophy of the charcot-marie-tooth type. It is an autosomal dominant disorder of lower motor neurons characterized by distal muscle weakness.

Source

Escherichia coli

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The HSPB8 protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Tris-acetate, pH-7.6, 10mM NaCl, 0.1mM EDTA, 0.1mM PMSF, 15mM ME.

Solubility

It is recommended to reconstitute the lyophilized HSPB8 in sterile H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized HSPB8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSPB8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Immunological Activity

Immunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSPB8.

References

1.Title: Roles of ERK and NF-?B in Interleukin-8 Expression in Response to Heat Shock Protein 22 in Vascular Smooth Muscle Cells.
Publication: Korean J Physiol Pharmacol. 2008 Aug;12(4): 171-176.
Link: http: //synapse.koreamed.org/DOIx.php?id=10.4196/kjpp.2008.12.4.171&vmode=FULL

Applications: HSP22 induces IL-8 in VSMCs via ERK1/2

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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HSPA5 Human, Hi-5, HSPB11 Human, HSPB7 Human, HSPB8 Human, His