Hepatitis C Virus�Combined, (Recombinant)

Recombinant Combined Hepatitis C Virus

Source: E.coli

Background: Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then contructed into one recombinant antigen. The protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions.

Purification method: Purified by proprietary chromatographic technique.

Purity: Recombinant Combined Hepatitis C Virus is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation: 1 mg/ml in50 mM Na-PO4, pH 8.5, 2.4 mM EDTA, 5 mM DTT and 0.1% SDS.

Storage: Recombinant Combined HCV is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability: Recombinant Combined HCV is stable 5 years frozen, One month in solution at room temperature.

Specificity: Immunoreactive with sera of HCV-infected individuals

Application: Recombinant Combined HCV Antigen may be used in ELISA and Western blots, excellent for detection of HCV with minimal specificity problems.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.