Hepatitis C Virus Nucleocapsid (core) GenoType-2b, (Recombinant)

Recombinant Hepatitis C Virus Nucleocapsid Genotype 2b (2-119 a.a)

Source: E.coli

Background: The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways In addition,HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.

Description: The protein contains the HCV core nucleocapsid immunodominant regions,amino acids 2-119. The protein is fused to a GST tag at N-terminus.

Purification method: Purified by proprietary chromatographic technique.

Purity: Recombinant HCV core 2 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation: 25mM Tris-HCl, pH 8.0, 1.5M Urea, 0.2% Triton-X and 50% glycerol.

Storage: Store at -20°C.

Stability: Recombinant HCV Core is stable 5 years frozen, One month in solution at room temperature.

Specificity: Immunoreactive with sera of HCV-infected individuals

Application: Recombinant HCV Core Antigen may be used in ELISA and Western blots, excellent for detection of HCV with minimal specificity problems.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.