HepatitisA VirusVP4-VP2 (Recombinant)

Recombinant Hepatitis A Virus VP4-VP2

Source: E.Coli

Background: HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5-kb single-stranded RNA genome bears different distinct regions: the 5' and 3' noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions.

Description: E.coli derived recombinant. The protein contains the HAV structural proteins VP2-VP4 immunodominant regions.

Purification method: Purified by proprietary chromatographic technique.

Purity: Protein is >90% pure as determined by 10% PAGE (coomassie staining).

Protein Conc.: 1mg/ml in 10mM CBB, pH9.6, 0.1% SDS, 50% glycerol.

Storage: Protein is shipped at ambient temperature. Upon arrival, Store at -200C.

Stability: Five years frozen. One month in solution at room temperature.

Specificity: Immunoreactive with sera HAV-infected individuals.

Application: Antigen in ELISA and Western blots, excellent antigen for detection of HAV with minimal specificity problems.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.