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Description

Recombinant Human HIV-I p66 pol

Source: Baculovirus

Description: Recombinant HIV-1 p66 pol (reverse transcriptase) has a Molecular weight of 66,000 dalton, glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells.

Physical Appearance: Sterile Filtered clear solution.

Formulation: Each ml of protein solution (100 g) contains 10mM Tris pH 8, 140mM sodium chloride and 400mM L-Arginine. .

Stability: Recombinant HIV-Ip66 although stable at 40C for 3 weeks, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological activity: Immunoreactive with sera from HIV-1 p66pol infected individuals. Western blots: 0.1-1.0 g/strip.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of HIV-1.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Properties

Storage Temperature

-20°C