Human Acetyl-Coenzyme A acetylTransferase 2 (Recombinant)

Recombinant Human Acetyl-Coenzyme A acetyltransferase 2 (ACAT2)

Source: E.Coli

Background: Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3 prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Description: Recombinant Human ACAT-2 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-397 and having a total molecular mass of 41.3 kda. Recombinant ACAT2 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear solution.

Formulation: Recombinant human Acetyl-Coenzyme A acetyltransferase 2 at a concentration of 0.1mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.

Stability: Recombinant human ACAT-2 although stable at 140C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Applications: ELISA MS Inhibition Assays Western Blotting

Purity: Greater than 95.0% as determined by the analysis by reducing and non-reducing SDS-PAGE coomassie blue staining.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.