Human Active Focal Adhesion Kinase (Recombinant)

Recombinant Human Active Focal Adhesion Kinase (FAK)

Source: Baculovirus, SF9 insect cells

Background: Focal adhesion kinase (FAK) is a critical mediator of signal transduction by integrins and growth factor receptors in a variety of cells including endothelial cells (ECs).It is a nonreceptor protein tyrosine kinase involved in signaling downstream of integrins, linking bacterial detection, cell entry, and initiation of proinflammatory response through MAPKs and NF-kappaB activation.

Description: Recombinant human FAK (amino acids 2 to 1052) is produced in Sf9 cells with an amino-terminal polyhistidine tag and purified via sequential chromatography.

Formulation: rHuFAKis supplied at a concentration of 0.1mg/ml in 40mM Tris, pH 7.5, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.1mM EGTA, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate, and 0.03% Brij-35.

Storage: Store at 4 C if entire vial will be used within 1-2 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 95% (SDS-PAGE).

Biological Activity: Recombinant Human FAK contains14units/mg. One unit of FAK activity transfers 1 nmole of phosphate to poly [GluTyr] substrate per minute at 30 C in a reaction mixture containing 8mM ATP. Substrates for FAK include STAT, paxillin, p85 PI 3-kinase, Cas, and tensin.FAK also undergoes autophosphorylation. Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.