Human Alanyl t-RNA Synthetase (Recombinant)

aRecombinant Human Alanine tRNA Ligase (rHuPL-12)

Source: Sf9 insect cells.

Background: Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.

Description: Recombinant Human PL-12 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 dalton. Recombinant PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant Human PL-12 is supplied at a of 0.9mg/ml in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% Glycerol.

Stability: Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 90% (SDS-PAGE).

Immunological functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).

Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.

Coating concentration: 0.3-0.8 g/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.