Human Alpha-Synuclein (Recombinant)

Recombinant Human alpha-Synuclein

Source: E.Coli

Background: alpha-Synuclein (amino acids 1-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. a-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Moreover, recent studies have shown that a-synuclein has chaperone activity and that this activity is lost upon removing its C-terminal acidic tail (amino acids 96-140).

Description: a-Synuclein Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain of 140 amino acids having a molecular mass of 14.4kDa(Real molecular weight on SDS-PAGE will be shift up).
The Recombinant Human a-Synuclein is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile filtered clorless solution.

Formulation: The protein (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl and 1mM MgCl₂

Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by SDS-PAGE.

Sequence: MDVFMKGLSK/ AKEGVVAAAE/ KTKQGVAEAA/ GKTKEGVLYV/ GSKTKEGVVH/GVATVAEKTK/ EQVTNVGGAV/VTGVTAVAQK/TVEGAGSIAA/ ATGFVKKDQL/ GKNEEGAPQE/ GILEDMPVDP/DNEAYEMPSE/EGYQDYEPEA.

Other Recombinant forms available: a-Synuclein A30P a-Synuclein A53T a-Synuclein A30P/A53T a-Synuclein Delta-NAC a-Synuclein 112(NACP112) a-Synuclein 61-140 a-Synuclein 96-140 a-Synuclein 1-60 a-Synuclein E46K a-Synuclein 1-95

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.