Human AminoAcylase-1 (Recombinant)

Recombinant Human AminoAcylase-1 (ACY1)

Source: E.Coli

Background: Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Description: Recombinant Human ACY-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-408 and having a total molecular mass of 46.9 kda. Recombinant Human ACY1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant Human Aminoacylase-1 at a concentration of 0.1mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.

Stability: Recombinant ACY-1 although stable at 140C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Applications: ELISA MS Inhibition Assays Western Blotting

Purity: Greater than 95.0% as determined by the analysis by reducing and non-reducing SDS-PAGE coomassie blue staining.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.