Human Artemin (Recombinant)

Recombinant Human Artemin - ART

Source: Baculovirus

Description: Recombinant Human Artemin produced in E.Coli is a disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 x 113 amino acids and having a total molecular mass of 24213 Daltons . Artemin is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant Artemin was lyophilized after extensive dialysis against 10mM sodium citrate pH-4.5 and 25mM sodium chloride.

Solubility: It is recommended to reconstitute the lyophilized Human Artemin in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Human Artemin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Murine C-10 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Gly-Gly-Pro-Gly.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: Artemin is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant proliferation assay SH-SY5Y cell line which was found to be 4-8ng/ml. The activity can also determined by it\'s ability to promote survival and neurite outgrowth.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human Artemin.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Artemin as a Reference Standard.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.