Human B-Cell Leukemia/Lymphoma 2 -BH4 (Recombinant)

Recombinant Human Bcl-2

Source: E. Coli

Description: Recombinant Human Bcl-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-218. Recombinant Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered colorless solution.

Formulation: The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl. Resuspend Bcl-2 in 100ul of 10% Acetic acid (Glacial), over night at 4C.

Stability: Recombinant Bcl-2 although stable at 120C for 1 week, should be stored desiccated below -180C. Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Other available Variants: Recombinant Human Bcl-2 minus BH1 domain Recombinant Human Bcl-2 minus BH2 domain Recombinant Human Bcl-2 minus BH3 domain Recombinant Human Bcl-2 minus BH4 domain, minus (10-30) Recombinant Human Bcl-2 NWGR Recombinant Human Bcl-2 N-terminal 8 kDa Recombinant Human Bcl-2 N-terminal + C-terminal Recombinant Human GST-Bcl-XL Recombinant Human Bcl-XL

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Bcl-2.

Applications: Input marker or positive control (Western Blotting). Function study (binding assay).

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.