Human B-Type Natriuretic Protein (Recombinant)

Recombinant Human Brain Natriuretic Peptide

Source: E.Coli

Background: Brain Natriuretic Protein is a protein secreted by the brain and the heart atria, stored mainly in cardiac ventricular myocardium. It can cause Natriuresis; Diuresis; Vasodilation; and inhibits secretion of Renin and Aldosterone.

Description: Recombinant Human Brain Natriuretic Peptide produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 32 amino acids, having a molecular mass of 3464 kDa. Human Brain Natriuretic Peptide is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Human Brain Natriuretic Peptide was lyophilized from 0.4ml PBS buffer containing 20mM phosphate buffer and 0.6mM sodium chloride.

Solubility: It is recommended to reconstitute the lyophilized Human BNP in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Human BNP although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Human BNP should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: Human Brain Natriuretic Peptide is fully biologically active when compared to standards.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human BNP.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of Human BNP as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.