Human Carbonic Anhydrase III (Recombinant)

Recombinant Human Carbonic Human Anhydrase III

Source: E.Coli

Description: Recombinant Human CAIII, Carbonic anhydrase III having a Mw of 33.9kDa is one of a group of ten zinc metalloenzymes that catalyse the reversible hydration of CO2 to form HCO3-. CAIII is a cytoplasmic isoenzyme, but is released into the circulation following injury. Recombinant human CAIII is expressed with an amino-terminal hexahistidine tag.

Physical Appearance: Sterile Filtered blue solution.

Formulation: CAIII Supplied in 1x Laemmli Buffer: 25mM Tris-HCl pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue and 2.5% Glycerol.

Stability: Human CAIII although stable at 14 C for 1 week, should be stored desiccated below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of CAIII.

content: Protein quantitation was carried out by two independent methods: (1) 1. UV spectroscopy at 280 nm. (2) Analysis by RP-HPLC, using a standard solution of CAIII as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.