Human Centromere Protein-B (Recombinant)

Synonyms

Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.

Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.

Description

CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 Dalton.CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Sf9 insect cells.

Physical Appearance

Sterile Filtered liquid formulation.

Formulation

CENPB is supplied in 20mM HEPES buffer pH-7.6, 250mM sodium chloride, and 20% glycerol.

Immunological functions

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Coating concentration

0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

CENPA Human