Human Chorionic Gonadotropin

Human Chorionic Gonadotropin (hCG)

Source: Urine of post-menopausal women

Description: hCG is produced from a sterile preparation of placental glucoprotein urine of post-menopausal women having a total molecular mass of 36,700 Dalton. hCG consists of 237 amino acids, chain-92 amino acids and chain-145 amino acids. The hCG is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.

Solubility: It is recommended to reconstitute the lyophilized hCG in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution hCG should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Biological Activity: ProSpec's hCG is biologically active compared to standard (IRP reference preparation 75/551). The activity was found to be 5000 IU/mg.

Contaminants: Free of: HbsAg, Hepatitis B surface antigen and antibodies to HIV, Hepatitis C and HCV.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of hCG as a Reference Standard.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.