Human Creatine Kinase MB Isoenzyme Type-2 (Recombinant)

Recombinant Human Creatine Kinase MB Isoenzyme Type-II

Source: Pichia Pastoris.

Background: The three isoenzymes (MM, MB, and BB) are found in muscle, cardiac and brain tissues. These recombinant proteins are ideal for calibrating diagnostic instruments and researching neuromuscular diseases. Creatine Kinases can be used for indications in many neuromuscular applications. These disorders include cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle's disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases, and other that create elevated or reduced levels of Creatine Kinases.

Description: Recombinant Human Creatine Kinase CKMBITII produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MB Isoenzyme in ELISA. Recombinant Human Creatine Kinase CKMBITII is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered colourless liquid formualtion.

Formulation: Each mg of protein (15.8mg/ml) contains 20mM Tris-HCl pH-7, 1mM EDTA and 1mM DTT.

Stability: Recombinant Human CKMBITII although stable at 150C for 7 days, should be stored desiccated below -180C. Please avoid freeze-thaw cycles. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 mole creatine phosphate was 500IU/mg at 37 degrees celsius. Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human CKMBITII.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.