Human D4-GDI (Recombinant)

Recombinant Human rho-GDI (D4-GDI)

Source: E.Coli

Background: D4-GDI belongs to the family of rho-GDI or GDP-dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of rhoGDI-1, D4-GDI/rhoGDI-2 and rhoGDI-3. It inhibits the release of GDP from the Rho proteins, essential for GTP-loading and activation of GTPase activity. D4-GDI is located in the cytoplasm and in involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas-mediated apoptosis

Description: Recombinant rho-GDI shows a band at 54kDa on SDS-PAGE commassie blue stained gel. Recombinant rho-GDI is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile filtered liquid.

Formulation: 100 g/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability: Store vial at -20Co to -80Co. When stored at the recommended temperature, D4-GDI is stable for 12 months. Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates: Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human D4-GDI.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.