Human ELK-1 (Recombinant)

Recombinant Human ELK1

Source: E.Coli

Description: Elk-1 is a transcription factor that is activated by mitogen-activated protein kinases (MAPKs) and whose main function is the regulation of growth-related proteins in response to extracellular stimuli. Because of the importance of Elk-1 to the regulation of successful cell growth, accurate monitoring of Elk-1 in cells, tissues and animals is crucial for many biomedical research and drug development projects.

Description: Recombinant Human ELK-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing 122 amino acids (307-428) and having a molecular mass of 60 kDa. This protein is conjugated with N-terminal His-tag and C-terminal myc tag (EQKLISEEDL). Recombinant Elk-1 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant Human ELK-1 is supplied as lyophilized freeze dry powder without additives.

Biological Activity: : Recombinant human Elk-1 is phosphorylatable in vitro, using either recombinant active ERK1/2, or with immunoprecipitated ERK1/2 from stimulated cells. This phosphorylation can be monitored by Western blot analysis using BioSource\\\'s phosphorylation site specific antibody directed to Elk-1 [pS383] in conjunction with chemiluminescence detection methods. Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Storage: Store at 4 C if entire vial will be used within 1-2 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles

Purity: Greater than 95% (SDS-PAGE).

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.