Human ERK1/MAPK3 Inactive Enzyme (Recombinant)

Recombinant Human ERK1/MAPK3 -Inactive Enzyme

Source: E.Coli

Description: Recombinant ERK1/MAPK3 is a highly active form produced by phosphorylation of the purified ERK1/MAPK3 in vitro with MEK1 is a non-glycosylated polypeptide having a molecular mass of 43.6kDa. Recombinant ERK1/MAPK3 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant Human ERK1 is supplied as 2.6mg/ml containing 50 mM Tris-HCL, 150mM NaCl, 2mM DTT, 5 mM EDTA, pH 8.0.

Unit definition: No protease activity detectable, specific activity > 200 U*/mg (*1 U = 1 pmol/min transferred to myelin basic protein at 30 degree C). No protease activity detectable.

Storage: Recombinant ERK1/MAPK3 should be stored at 4 C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20 C. Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: None.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.