Human ERK2/MAPK1 Active Enzyme (Recombinant)

Recombinant Human ERK2/MAPK1 (p42 MAP Kinase) -Active Enzyme

Source: E.Coli

Description: Recombinant ERK2 (extracellular signal-regulated kinase) a Mitogen-Activated Protein Kinase, is a highly active form produced by phosphorylation of the purified ERK2/MAPK1 in vitro with MEK1 is a non-glycosylated polypeptide chain containing amino acids 2-480 and having a molecular mass of 44.6kDa. Recombinant ERK2/MAPK1 contains an amino terminal polyhistidine tag and is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant Human ERK2 is supplied as 0.16mg/ml containg 50 mM Tris-HCL, 150mM NaCl, 1mM DTT, 50% glycerol, pH 8.5.

Unit definition: 1 U = 1 pmol/min transferred to myelin basic protein at 30 dego C.

Storage: Recombinant ERK2/MAPK1 should be stored at 4 C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20 C. Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: specific activity > 370 U/mg.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.