Human Fibroblast Growth Factor-22 (Recombinant)

Recombinant Human Fibroblast Growth Factor-22 (FGF-22)

Source: E.Coli

Background:

Description Recombinant Human FGF-22 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids and having a molecular mass of 17173 Dalton. Recombinant FGF-22 is purified by chromatographic techniques.

Physical Appearance: Sterile Filtered white lyophilized powder.

Formulation: The sterile protein powder is lyophilized with no additives.

Solubility: It is recommended to reconstitute the lyophilized Recombinant Human FGF-22 sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuFGF-22 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rHuFGF-22 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Pro-Ser-Ala-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: Measured in a cell proliferation assay using IEC6 cell line (20ng/ml can stimulate the cell proliferation of IEC6 cell line).

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant Human FGF-22.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.