Human FormiminoTransferase Cyclodeaminase (Recombinant)

Recombinant Human Formiminotransferase Cyclodeaminase (LC-1)

Source: Sf9 insect cells.

Background: Formiminotransferase cyclodeaminase, a bifunctional enzyme of tetrahydrofolate synthesis, is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis (for which anti-LKM 1/cytochrome P450 2D6 autoantibodies are a further marker). Serum LC1 autoantibody concentrations appear to fluctuate in parallel with aminotransferase levels, a particularly intriguing observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanism leading to hepatocyte injury.

Description: Recombinant Human Formiminotransferase Cyclodeaminase 1 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 59,749 dalton. Recombinant LC-1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance: Sterile filtered liquid formulation

Formulation: Recombinant Human LC-1 is supplied at a of 0.5mg/ml in 16mM HEPES buffer pH-7.6, 240mM sodium chloride, and 20% glycerol.

Immunological functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).

Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody.and autoimmune hepatitis (AIH II) patient sera.

Coating concentration: 0.35-0.7 g/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Storage Conditions: Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 Cfor longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 95% (SDS-PAGE).

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.