Human Glucagon Like Peptide-1 (7-36), (Recombinant)

Recombinant Human Glucagon Like Peptide-1/7-36 (GLP-1)

Source: E.coli

Background: GLP-1 is a incretion hormone, which has been shown to stimulate secretion of insulin in the presence of elevated blood glucose concentrations, but not during periods of low blood glucose concentrations (hypoglycemia). GLP-1 is released from gut endocrine cells in response to nutrient ingestion. The hormone is a physiologic mediator of satiety and regulates energy absorption and disposal. GLP-1 is an insulinotropic hormone that has the ability to render pancreatic beta cells glucose-competent. It has been used in the therapy of type-2 diabetes. GLP-1 has cytokine activity, although it is not classified as such due its small size GLP-1 enhances cell differentiation, plays a role in tissue regeneration, and mediates cytoprotection.

Description: Recombinant Human Glucagon Like Peptide-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 30 amino acids and having a molecular mass of 3298.7Dalton. Human GLP-1 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: GLP-1 (1mg/ml) was lyophilized after extensive dialyses against 0.1 mg sodium phosphate monobasic & 1.6mg sodium phosphate dibasic.

Solubility: It is recommended to reconstitute the lyophilized Human GLP-1 in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Human GLP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Human GLP-1 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Hiis-Ala-Glu-Gly-Thr.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of GLP-1 as a Reference Standard.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human GLP.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.