Human Glycogen Phosphorylase (Recombinant)

Recombinant Human Glycogen Phosphorylase (GPBB)

Source: E.Coli

Background: Glycogen Phosphorylase is an enzyme that catalyzes the degradation of Glycogen in animals by releasing glucose-1-phosphate from the terminal alpha-1,4-glycosidic bond. This enzyme exists in two forms: an active phosphorylated form ( Phosphorylase A) and an inactive un-phosphorylated form (Phosphorylase B). Both a and b forms of phosphorylase exist as homodimers. In mammals, the major isozymes of glycogen phosphorylase are found in muscle, liver and brain tissue. Glycogen Phosphorylase- a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. Glycogen Phosphorylase can also be used as a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.

Description: Recombinant Human Glycogen Phosphorylase produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 97 kDa. Recombinant Glycogen Phosphorylase is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile filtered liquid formulation

Formulation: 0.61mg/1ml. Each mg of protein contains 50% glycerol.

Stability: Recombinant Glycogen Phosphorylase, although stable at 10C for 1 week, should be stored desiccated below -18 C. Please avoid freeze-thaw cycles.

Purity: Greater than 85.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Applications: Immunoassays and western blot.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant Glycogen Phosphorylase.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.