Human GRO-alpha (CXCL1), (Recombinant)

Recombinant Human GRO-Alpha/MGSA (CXCL1)

Source: E.Coli

Background: Three human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation. Melanoma growth stimulatory activity/gro alpha (MGSA), a member of the alpha-chemokine family, is produced by a variety of dermal and epidermal cells and can act in a paracrine and autocrine fashion.

Description: Recombinant Human GRO-alpha produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7811 Dalton. Recombinant Human GRO-alpha is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized with no additives.

Solubility: It is recommended to reconstitute the lyophilized GRO-alpha in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized MGSA/GRO-a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MGSA/GRO-alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ser-Val-Ala-Thr.

Dimers and aggregates: Less than 1% as determined by silver stained SDS-PAGE gel analysis.

Biological Activity: Recombinant Melanoma growth stimulatory activity is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human neutrophils cells using a conc. of 10.0-50.0 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of GRO-alpha .

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of MGSA as a Reference Standard.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.