Human Growth Hormone (Recombinant)

Recombinant Human Growth Hormone (GH)

Synonym: GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.

Source: E.Coli

Description: Recombinant HGH produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22260.01 Dalton. Recombinant HGH is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The GH (1mg/ml) was lyophilized after extensive dialyses against 8mg mannitol and 0.34mg sodium phosphate buffer (0.02 mg sodium phosphate monobasic & 0.32mg sodium phosphate dibasic).

Solubility: It is recommended to reconstitute the lyophilized Growth Hormone in sterile 18MO-cm H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Recombinant GH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGH can be stored at 4°C for 2-7 days and below -18°C for longer. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ile.

Biological Activity: Recombinant Human Growth Hormone is fully biologically active when compared to World Health Organization (WHO) reference standard which is 3 units/mg corresponding to a specific acitivity of 333,334ng/ml.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.72 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.