Human HCC-1 (CCL14), (Recombinant)

Recombinant Hemofiltrate CC-Chemokine-1 (hcc-1)

Source: E.Coli

Description: Recombinant Human HCC-1 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8411 Dalton. Human HCC-1 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Human HCC-1 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility: It is recommended to reconstitute the lyophilized Human HCC-1 in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized HCC-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution HCC-1 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Glu-Ser-Ser-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: bioWORLD\'s HCC-1 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract Human monocytes at 5.0-20.0 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human HCC-1.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of HCC-1 as a Reference

Usage: bioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.