Human Histidyl-tRNA Synthetase (Recombinant)

Recombinant Human Histidyl tRNA synthetase (JO-1)

Source: E.Coli

Description: Recombinant Human Histidyl tRNA synthetase (JO-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 55kDa. Recombinant Histidyl tRNA synthetase is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear solution.

Formulation: Recombinant JO-1 (1.2mg/ml) contains 150mM NaCl and 10mM sodium phosphate containing 0.1% NaN3 (pH 7.2).

Stability: JO-1 although stable at 4oC for 3 weeks, should be stored desiccated below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human Histidyl tRNA synthetase .

Protein content: Protein quantitation was carried out by: 1. 0.25 - 2.0 mg/ml Bradford assay vs. BSA.

Western blot: Strongly reactive with human anti JO-1antisera.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.