Human Insulin Like Growth Factor Binding Protein-3 (Recombinant)

Recombinant Human Insulin Like Growth Factor Binding Protein-3 (IGFBP-3)

Source: E.Coli

Background: Insulin Like Growth Factor Binding Protein-3 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. IGFBP-3 is the major IGF binding protein present in serum of humans and animals. It is also present in the alpha granules of platelets. IGFBP-3 shows a similar affinity for IGF-1 and IGF-2 . IGF-BP-3 inhibits follicle stimulating hormone . Markedly decreased levels of IGF-BP-3 are observed in patients with Growth hormone deficiencies, while markedly elevated levels are observed in patients with high levels of Growth hormone (acromegaly). In murine fibroblasts the synthesis of IGFBP-3 is stimulated by mitogenic growth factors such as Bombesin , Vasopressin , PDGF , and EGF . In human skin fibroblasts the synthesis of IGFBP-3 is stimulated by TGF-beta .

Description: Recombinant Human IGFBP-3 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 264 amino acids and having a molecular mass of 28806 Dalton. IGFBP-3 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: IGFBP-3 was lyophilized with 20mM Tris HCL .

Solubility: It is recommended to reconstitute the lyophilized IGFBP-3 in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized IGFBP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IGFBP-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ala-Ser-Ser-Gly.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: IGFBP-3 is fully biologically active when compared to standards. The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is less then 0.2 g/ml in the presence of 15 ng/ml of Human IGF-II.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of IGFBP-3 .

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of IGFBP-3 as a Reference Standard.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.