Human Interferon-beta 1b (Recombinant)

Recombinant Human Interferon-beta 1b

Synonym: Leukocyte interferon, B cell interferon, Type I interferon, IFNB1, IFB, IFF, IFNB, IFN-b 1b, MGC96956.

Source: E.Coli

Background: One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 .

Description: Recombinant Human IFN-beta 1b produced in E.Coli is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton. The Interferon beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17. IFN-beta-1b is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: 1mg/ml solution containing 50mg Human Albumin & 50mg dextrose.

Solubility: It is recommended to reconstitute the lyophilized Interferon beta-1b in sterile 18MO-cm H₂O not less than 100 μ/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Interferon beta-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, liquid form of IFN-beta-1b can be stored at 4°C for up to 3 weeks and -18°C for longer. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.

Biological Activity: Interferon beta-1b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32 x 10⁶ IU/mg.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IFN-beta as a Reference Standard.