Human Interleukin-1 alpha (Recombinant)

Recombinant Human Interleukin-1 alpha (IL-1 alpha)

Source: E.Coli

Background: A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL1-alpha and TNF-alpha induce the synthesis of IL1-beta . In pheochromocytoma cells, NGF induces the synthesis of IL1.

Description: Recombinant Human IL-1 produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton. The rHuIL-1&alpha is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM Tris-HCL, pH=8, 5mM MgCl2 and 10% glycerol.

Solubility: It is recommended to reconstitute the lyophilized rHuIL-1&alpha in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuIL-1&alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-1&alpha should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Pro-Phe-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: rHuIL-1&alpha is fully biologically active when compared to standards. The ED50 as determined by the dose-dependant stimulation of murine D10S cells is less then 0.001 ng/ml, corresponding to a Specific Activity of 1 x 10 IU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of rHuIL-1&alpha.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of chicken IL-1&alpha as a Reference Standard.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.