Human Interleukin-10, CHO (Recombinant)

Recombinant Human Interleukin-10 (IL-10)

Source: CHO

Background: Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice are Ly-1 B-cells that express CD5 and CD11 . Murine keratinocytes also produce IL10. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4 (+) T-cell clones (resembling Th0 , Th1 , and Th2 ) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cell lines derived from patients with acquired immunodeficiency syndrome and Burkitt\\\'s lymphoma constitutively secrete large quantities of IL-10 into the conditioned medium . The synthesis of IL-10 by monocytes is inhibited by IL-4 and IL-10.

Description: Recombinant Human IL-10 produced in CHO is a single, glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18606.29 Dalton. Recombinant IL-10 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant Human Interleukin-10 was lyophilized from a concentrated (1mg/ml) solution containing 60mM PBS pH-6.5 and 100mM NaCl .

Solubility: It is recommended to reconstitute the lyophilized IL-10 in sterile 18M?-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-10 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: Interleukin-10 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 107 IU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of IL-10.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a standard solution of IL-10 as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.