Human Interleukin-20 (Recombinant)

Recombinant Human Interleukin-20 (IL-20)

Source: E.Coli

Background: The newly discovered cytokine interleukin-20 (IL-20) is structurally related to IL-10, yet it appears to be an autocrine factor for keratinocytes that regulates their participation in inflammation.

Description: Recombinant Human IL-20 produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing 2 x 153 amino acids and having a total molecular mass of 35,212 Dalton. Recombinant IL-20 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant hIL-20 was lyophilized from a concentrated (1mg/ml) solution containing 100mM sodium chloride and 10mM sodium citrate buffer pH-3.

Solubility: It is recommended to reconstitute the lyophilized Interleukin-20 in sterile 18M?-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized IL-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rhIL-20 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Lys-Thr-Leu.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: rHuIL-20 is fully biologically active when compared to standard. The Biological Acitivity was determined by the ability to activiate STAT following receptor ligand interaction.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of hIL-20.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of IL-20 as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.