Human Intrinsic Factor (Recombinant)

Recombinant Human Intrinsic Factor (IF)

Source: Sf9 Insect Cells

Description: Recombinant Human Intrinsic Facor (IF, GIF, Cobalamin/Vitamin B-12 binding transport protein). Recombinant Human IF produced in baculovirus is a glycosylated, polypeptide chain containing having a molecular mass of 44,228 Dalton. Human IF is fused to a hexa-histidine at the C-terminus and purified by proprietary chromatographic techniques for removal of bound Vitamin B-12.

Physical Appearance: Sterile Filtered colorless solution.

Formulation: The protein solution contains 20mM HEPES pH-8.

Stability: Recombinant IF although stable at 100C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Biological Activity: Standard ELISA test (checker-board analysis of positive/negative sera panels), immunodot with anti-IF autoantibody positive patient sera. Can be used for solid ELISA and fluid phase diagnostic assays, 0.25-0.65 g/ml. Suitable for biotinylation and iodination.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant Human IF.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.