Human Keratin-8 (Recombinant)

Recombinant Human Keratin-8

Source: E.Coli

Background: Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.

Description: Recombinant Keratin-8 shows a band at 78kDa on SDS-PAGE commassie blue stained gel. Recombinant Keratin 8 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile filtered liquid.

Formulation: 100 g/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability: Store vial at -20Co to -80Co. When stored at the recommended temperature, recombinant Keratin-8 is stable for 12 months. Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates: Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human Keratin.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.